Get the latest tech news
Claustrum neurons projecting to the anterior cingulate restrict engagement
Attention and sleep have been associated with the claustrum. Here authors pinpoint a cluster of neurons in the claustrum of male mice that dynamically determines engagement levels during both sleep and an attentional task.
To this end, we recorded the activity of claustrum neurons that project to the anterior cingulate cortex (ACCp) using calcium fiber photometry in unrestrained mice, while simultaneously performing polysomnography (video, EEG – electroencephalography and EMG – electromyography) to determine their sleep state (Figs. Simultaneous monitoring of the two channels was made possible by connecting the LEDs to a 4-port minicube (with dichroic mirrors and cleanup filters to match the excitation and emission spectra; FMC4 or FMC5, Doric Lenses) via an attenuating patch cord (400 µm core, NA = 0.37-0.48). After baseline correction, ΔF/F was computed using the 1st lowest percentile value as F0 (\(\frac{\Delta F}{F}=\frac{F-{F}_{0}}{{F}_{0}}\)), and the resulting trace was z-scored relative to the mean and standard deviation of the entire recording session to normalize across channels, indicators, and mice.
Or read this on Hacker News