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External RF-EMF Alters Cell Number and Reactive Oxygen Species Balance


The influence of weak radio-frequency electromagnetic field (RF-EMF) on living organisms raises new concern because of the Industrial, Scientific, and Medica...

A recent study has shown that a weak external RF-EMF (10 μT at 7 MHz) could alter cell growth and the level of reactive oxygen species (ROS) ( 1), but the underlying molecular mechanisms remain elusive. HUVEC were cultured in humidified incubator overnight followed by exposure of RF-EMF (10 μT at 6.78 MHz) for 24 h. For O 2 •- assay, cells were stained with ROS red working solution for 1 h. Fluorescence intensity at 605 nm was measured using VICTOR multilabel plate reader. High level of NADPH not only helps supporting the biosynthesis of essential materials for rapid cell growth, but also promotes the generation of reduced forms of antioxidant molecules that power up the redox defense ( 25, 26).

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